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Plant responses to combined arsenic and cadmium stress
Burešová, Andrea ; Mašková, Petra (advisor) ; Podlipná, Radka (referee)
Arsenic and cadmium are both non-essential, highly toxic and carcinogenic elements that often occur together in the environment. Given the frequent co-contamination of the environment, it is necessary to investigate both plant strategies for dealing with one metal(loid) and the mechanisms that lead to tolerance or, conversely, sensitivity in the presence of both elements. Simultaneous exposure to multiple toxic elements may lead to extensive plant damage, however it may also result in the increasing engagement and intensity of defence strategies. Contamination of the food chain through crops growing on contaminated soils is a major concern, one that poses a risk to both human and animal lives. The aim of this research field is to reduce accumulation and translocation to aboveground edible parts as much as possible, for example by understanding the mechanisms behind heavy metal accumulation and translocation, or by stabilising toxic elements in the soil, or by cleaning up contaminated soil. One method of clean-up is phytoremediation, which usually utilizes plants with high tolerance to toxic elements, called hyperaccumulators. The knowledge of how defence strategies and mechanisms are affected by the interaction of multiple elements is important for identifying plant species capable of activating...
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The application of Brdička reaction for determination of phytochelatins in real samples of barley and thermophilic cyanobacterium grown in the presence of cadmium ions
Čížková, Petra ; Fedjuk, K. ; Šestáková, Ivana
Heavy metals represent important group of environmental contaminants. Ions of heavy metals (Cd, Pd, Cu, Hg, Zn and Ni) are released into soil solutions as a consequence of soils acidification, among others. Their presence has imminent influence on physiological procedures in plants, which have developed different mechanisms of adaptation on such conditions. One of mechanisms for plants tolerance towards heavy metal ions is their inactivation by binding on polypeptides or small proteins with high cysteine content, named phytochelatins (PC) or metallothioneins. Their function has been studied in particular at man and animals.
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Phytochelatins as Cadmium and Zinc Ligands in Study Using Voltammetric Methods
Šestáková, Ivana ; Navrátil, Tomáš
Complexation of synthetically prepared phytochelatin PC3 was studied using differential pulse voltammetry on HMDE. This method allows to measure reduction peak of mercury compound of original peptide simultaneously with the reduction peaks of formed cadmium or zinc complexes. Elimination voltammetry with linear scan was used at selected stoichiometric ratio to characterise diffusion current of complexes formed. Substantial differences were found between Cd and Zn complexes, both in formation and behaviour.
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